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황우석 연구팀, 세계 최초 돼지복제 줄기세포 수립

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황우석 연구팀, 세계 최초 돼지복제 줄기세포 수립
Zygote, Volume 18, Issue 02, May 2010, pp 93-101

황우석박사가 이끄는 연구팀 수암연구재단이 세계 최초로 포유류인 돼지 복제 줄기세포 수립에 성공하였습니다. 이 기술은 인간 체세포 복제줄기세포를 만드는 데도 적용될 수 있습니다. 영국 케임브리지大 출판사에서 발행하는 국제학술지 'zygote'에 이 내용을 담은 논문은 황우석 연구팀이 인간이나 동물줄기세포에 대한 독자적 연구가 가능해졌음을 시사합니다.

'zygote'

Establishment and characterization of embryonic stem-like cells from porcine somatic cell nuclear transfer blastocysts

S. Kim, J.H. Kim, E. Lee, Y.W. Jeong, M.S. Hossein, S.M. Park, S.W. Park, J.Y. Lee, Y.I. Jeong, H.S. Kim, Y.W. Kim, S.H. Hyun and W.S. Hwang

Zygote, Volume 18, Issue 02, May 2010, pp 93-101
doi:10.1017/S0967199409990372 (About doi), Published Online by Cambridge University Press 22 Mar 2010

Summary
This study was aimed to establish embryonic stem (ES)-like cells from blastocysts derived from somatic cell nuclear transfer (SCNT) in pig. Somatic cells isolated from both day-30 fetus and neonatal cloned piglet were used for donor cells. A total of 60 blastocysts (46 and 14 derived from fetal and neonatal fibroblast donor cells, respectively) were seeded onto a mitotically inactive mouse embryonic fibroblast (MEF) monolayer and two ES-like cell lines, one from each donor cell type, were established. They remained undifferentiated over more than 52 (fetal fibroblast-derived) and 48 (neonatal fibroblast-derived) passages, while retaining alkaline phosphatase activity and reactivity with ES specific markers Oct-4, stage-specific embryonic antigen-1 (SSEA-1), SSEA-4, TRA-1-60 and TRA-1-81. These ES-like cells maintained normal diploid karyotype throughout subculture and successfully differentiated into embryoid bodies that expressed three germ layer-specific genes (ectoderm: beta-III tubulin; endoderm: amylase; and mesoderm: enolase) after culture in leukemia inhibitory factor-free medium. Microsatellite analysis confirm!!!ed that they were genetically identical to its donor cells. Combined with gene targeting, our results may contribute to developing an efficient method for producing transgenic pigs for various purposes.

(Received October 27 2008)

(Accepted April 22 2009)

(Online publication March 22 2010)

http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=7466480

美 국립의학도서관(National Library of Medicine) 자료 검색

http://www.ncbi.nlm.nih.gov/sites/entrez

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